Size: 934 acres
Date conserved: 2012 to present

Partner Organizations: Florida Communities Trust


Santa Fe River Preserve protects 934 acres on the banks of the Santa Fe River near Worthington Springs which includes high bluffs along the river and an interesting tangle of floodplain forest where the New River converges with the Santa Fe.

The preserve has an interesting mix of flora which in turn support a wide diversity of wildlife. There are wild azaleas and rain lilies on the banks; large birch trees, cypresses, and gums in the bottomlands; and a ridge of live oaks and pines in the uplands with lots of sparkleberry and wire grass in the understory. Bears frequent the river corridor, and there is a rare and endangered mussel in this watershed.

South (main) Entrance

27025 North State Road 121
Alachua, FL 32615

North Entrance

The North Entrance to the preserve is approximately 1.5 miles north of the South (Main) Entrance at the GPS coordinates of 29.916130, -82.426157 



• Public trails for hiking and bicycling (approx. 2.7 miles).
• Dogs are welcome.
• Trails are unpaved, and in some places can be muddy, uneven, or wet.
• Parking is available at trailhead.
• Preserve closes at dark.

Trail MAP (Click to download)